Australasian Plant Pathology (2006) 35, 147-159
A. Drenth, G. Wagels, B. Smith, B. Sendall, C. O'Dwyer, G. Irvine and J.A.G. Irwin (2006)
Development of a DNA-based method for detection and identification of Phytophthora species
Australasian Plant Pathology 35 (2), 147-159
Abstract: Phytophthora diseases cause major losses to agricultural and horticultural production in Australia and worldwide. Most Phytophthora diseases are soilborne and difficult to control, making disease prevention an important component of many disease management strategies. Detection and identification of the causal agent, therefore, is an essential part of effective disease management. This paper describes the development and validation of a DNA-based diagnostic assay that can detect and identify 27 different Phytophthora species. We have designed PCR primers that are specific to the genus Phytophthora. The resulting amplicon after PCR is subjected to digestion by restriction enzymes to yield a specific restriction pattern or fingerprint unique to each species. The restriction patterns are compared with a key comprising restriction patterns of type specimens or representative isolates of 27 different Phytophthora species. A number of fundamental issues, such as genetic diversity within and among species which underpin the development and validation of DNA-based diagnostic assays, are addressed in this paper.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Andre Drenth
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
